ARF4 Antibody

• Catalog Number: orb1248657
• Category: Antibodies
• Description: ARF4 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Bovine, Porcine, Rat
• Reactivity: Human, Mouse
• Immunogen: The immunogen for this antibody is: C-SEMTDKLGLQSLRN
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 19kDa band observed in lysates of cell lines NIH3T3 and HeLa (calculated MW of 20.5kDa according to Human NP_001651.1 and 20.4kDa according to Mouse NP_031505.1 ). Recommended concentration: 0.3-1ug/ml. Primary incubation 1 hour at room temperature.Immunohistochemistry:Paraffin embedded Human Thymus. Recommended concentration: 5ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Approx 19 kDa
• Target: ARF4
• UniProt ID: P18085
• NCBI: NP_001651.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: ARF4, ADP-ribosylation factor 4, ARF2, ADP-ribosyl
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 19kDa band observed in lysates of cell lines NIH3T3 and HeLa (calculated MW of 20.5kDa according to Human NP_001651.1 and 20.4kDa according to Mouse NP_031505.1 ). Recommended concentration: 0.3-1ug/ml. Primary incubation 1 hour at room temperature.Immunohistochemistry:Paraffin embedded Human Thymus. Recommended concentration: 5ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1248657 (0.5 ug/ml) staining of NIH3T3 (A) and HeLa (B) lysates (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1248657 (5 ug/ml) staining of paraffin embedded Human Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.