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Catalog Number | orb1264524 |
---|---|
Category | Antibodies |
Description | APRT Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC-P, WB |
Reactivity | Human, Rat |
Isotype | Rabbit Ig |
Immunogen | This APRT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 143-170 amino acids from the C-terminal region of human APRT. |
Concentration | batch dependent |
Dilution range | For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | 20 kDa |
Target | APRT |
UniProt ID | P07741 |
NCBI | P07741 |
Storage | Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Buffer/Preservatives | Supplied in PBS with 0.09% (W/V) sodium azide. |
Alternative names | Adenine phosphoribosyltransferase, APRT, APRT Read more... |
Note | For research use only |
Application notes | For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100 |
Expiration Date | 12 months from date of receipt. |
Western Blot at 1:1000 dilution Lane 1: K562 whole cell lysate Lane 2: MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in MCF-7 cell line and rat kidney tissue lysates (35 ug/lane).
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with APRT Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
IF, IH, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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