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Catalog Number | orb1239195 |
---|---|
Category | Antibodies |
Description | APP Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, IF, IHC-P, WB |
Predicted Reactivity | Guinea pig, Porcine |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | APP antibody was raised against a 15 amino acid peptide near the carboxy terminus of human APP.The immunogen is located within the last 50 amino acids of APP. |
Concentration | 1 mg/ml |
Dilution range | APP antibody can be used for detection of APP and the C99 fragment by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse and rat samples and Immunofluorescence in mouse and rat samples. All other applications and species not yet tested. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Predicted: 83 kDa Observed: 110 kDa |
Target | APP |
UniProt ID | P05067 |
NCBI | CAA30050 |
Storage | APP antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures. |
Buffer/Preservatives | APP Antibody is supplied in PBS containing 0.02% sodium azide. |
Alternative names | APP Antibody: AAA, AD1, PN2, ABPP, APPI, CVAP, ABE Read more... |
Note | For research use only |
Application notes | APP antibody can be used for detection of APP and the C99 fragment by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse and rat samples and Immunofluorescence in mouse and rat samples. All other applications and species not yet tested. |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of APP in mouse brain tissue lysate with APP antibody at (A) 0.5 and (B) 1 µg/ml.
Immunohistochemical staining of rat brain using APP antibody at 2 µg/mL.
Immunofluorescence of APP in Rat Brain cells with APP antibody at 20 µg/mL.
Immunohistochemistry of APP in mouse brain tissue with APP Antibody at 5 µg/mL.
Immunofluorescence of APP in mouse brain tissue with APP Antibody at 20 µg/mL. Green: APP antibody (orb1239195) Red: Phylloidin staining Blue: DAPI staining.
FC, ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, ICC, IF, IHC, IHC-Fr, WB | |
Bovine, Equine, Monkey, Rabbit | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IF, IHC, WB | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IF, IHC, WB | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
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