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Catalog Number | orb1929129 |
---|---|
Category | Antibodies |
Description | Purified Rabbit Polyclonal Antibody (Pab) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Clone Number | RB15180 |
Tested applications | IHC-P, WB |
Predicted Reactivity | Rabbit |
Reactivity | Human, Mouse |
Isotype | Rabbit IgG |
Antibody Type | Primary Antibody |
Dilution range | WB: 1:1000, IHC-P: 1:10~50 |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Conjugation | Unconjugated |
MW | 36154 Da |
Target | This APOE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 263-292 amino acids from the C-terminal region of human APOE. |
UniProt ID | P02649 |
NCBI | NP_000032.1 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | Apolipoprotein E, Apo-E, APOE Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with APOE antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
All lanes: Anti-APOE Antibody (C-term) at 1:1000 dilution. Lane 1: Human plasma lysate. Lane 2: Mouse plasma lysate. Lane 3: Human ovary lysate. Lane 4: Mouse liver lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 36 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.