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Catalog Number | orb1240222 |
---|---|
Category | Antibodies |
Description | APH1A Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, IHC-P, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | APH1 antibody was raised against a 18 amino acid synthetic peptide from near the center of human APH1.The immunogen is located within amino acids 80 - 130 of APH1. |
Concentration | 1 mg/mL |
Dilution range | APH1 antibody can be used for detection of APH1 by Western blot at 0.5 - 1 μg/mL. Despite its predicted molecular weight, APH1 protein often migrates at aberrant locations in SDS-PAGE. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. Antibody validated: Western Blot in mouse samples and Immunohistochemistry in human samples. All other applications and species not yet tested. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Predicted: 29 kDa Observed: 28 kDa |
Target | APH1A |
UniProt ID | Q96BI3 |
NCBI | AAH08732 |
Storage | APH1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Buffer/Preservatives | APH1 Antibody is supplied in PBS containing 0.02% sodium azide. |
Alternative names | APH1 Antibody: APH-1, APH-1A, CGI-78, 6530402N02Ri Read more... |
Note | For research use only |
Application notes | APH1 antibody can be used for detection of APH1 by Western blot at 0.5 - 1 μg/mL. Despite its predicted molecular weight, APH1 protein often migrates at aberrant locations in SDS-PAGE. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. Antibody validated: Western Blot in mouse samples and Immunohistochemistry in human samples. All other applications and species not yet tested. |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of APH1 in RAW264.7 cell lysate with APH1 antibody at (A) 1 and (B) 2 µg/mL.
Immunohistochemistry of APH1 in human brain tissue with APH1 antibody at 5 µg/mL.
ELISA, IF, WB | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IHC, WB | |
Bovine, Canine, Human, Mouse, Porcine, Rat | |
Goat | |
Polyclonal | |
Unconjugated |
ELISA, IHC, WB | |
Bovine, Canine, Mouse, Porcine, Rat | |
Human | |
Goat | |
Polyclonal | |
Unconjugated |
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