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Catalog Number | orb345453 |
---|---|
Category | Antibodies |
Description | Apg7 antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, IF, WB |
Reactivity | Human |
Isotype | IgG |
Immunogen | This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 550-575 of Human Apg7 protein. |
Concentration | 1.0 mg/mL |
Dilution range | ELISA: 1:300,000 - 1:400,000, IF: User Optimized, WB: 1:500 - 1:2,000 |
Form/Appearance | Liquid (sterile filtered) |
Purity | This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase. Reactivity occurs against human Apg7 protein. However, BLAST analysis indicates 100% homology (12/12) for this protein from human, chimpanzee, mouse, rat, dog, and chicken sources. The core amino acids of the immunogen, G-D-S-T-R-D-R-T are 100% identical (8/8) in D. melanogaster. Reactivity with Apg7 proteins from other sources is not known. |
Conjugation | Unconjugated |
UniProt ID | O95352 |
NCBI | NP_001129503.2 |
Storage | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Alternative names | rabbit anti-APG7 Antibody, APG-7, APG 7, APG7 auto Read more... |
Note | For research use only |
Application notes | This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 78 kDa in size corresponding to human Apg7 by western blotting in the appropriate cell lysate or extract. Human Apg7 has been reported to form a homodimer at approximately 160 kDa. |
Expiration Date | 12 months from date of receipt. |
IF, IH, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, ICC, IF, WB | |
Gallus, Rat | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
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