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Catalog Number | orb1274460 |
---|---|
Category | Antibodies |
Description | APEX1 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IP, WB |
Reactivity | Human |
Isotype | IgG |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-318 of human APEX1 (NP_542380.1). |
Concentration | batch dependent |
Dilution range | WB: 1:500 - 1:2000IF: 1:50 - 1:100IP: 1:50 - 1:200 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 39kDa |
Target | APEX1 |
UniProt ID | P27695 |
Storage | Store at -20˚°C. Avoid freeze / thaw cycles. |
Buffer/Preservatives | Liquid |
Alternative names | APEX1, APE, APX, APE1, APEN, APEX, HAP1, REF1 Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:2000IF: 1:50 - 1:100IP: 1:50 - 1:200 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using APEX1 antibody (orb1274460). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Western blot analysis of extracts from normal (control) and APEX1 knockout (KO) 293T cells, using APEX1 antibody (orb1274460) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunofluorescence analysis of HeLa cells using APEX1 antibody (orb1274460). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of A549 cells using APEX1 antibody (orb1274460).
Immunoprecipitation analysis of 200 ug extracts of HeLa cells using 1 ug APEX1 antibody (orb1274460). Western blot was performed from the immunoprecipitate using APEX1 antibody (orb1274460) at a dilution of 1:1000.
FC, ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ChIP, ELISA, IHC, WB | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
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