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Catalog Number | orb215883 |
---|---|
Category | Antibodies |
Description | APE1/APEX1 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | FC, ICC, IF, IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | Rabbit IgG |
Immunogen | E.coli-derived human APE1 recombinant protein (Position: P2-L318). Human APE1 shares 94% and 93% amino acid (aa) sequences identity with mouse and rat APE1, respectively. |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 35555 MW |
UniProt ID | P27695 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | DNA- (apurinic or apyrimidinic site) lyase;3.1.-.- Read more... |
Note | For research use only |
Application notes | WB: The detection limit for APE1 is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
Flow Cytometry analysis of U937 cells using anti-APEX1 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.
WB analysis of APEX1 using anti-APEX1 antibody.Lane 1:Recombinant Human APEX1 Protein 0.5ng.
WB analysis of APEX1 using anti-APEX1 antibody.Lane 1:NRK Cell;2:HELA Cell;3:PC-12 Cell;4:RH35 Cell;5:HEPA Cell;6:MCF Cell;7:A549 Cell;8:Human Placenta Tissue;9:A431 Cell.
IF analysis of APEX1 using anti-APEX1 antibody.APEX1 was detected in immunocytochemical section of U20S cell.
IF analysis of APEX1 using anti-APEX1 antibody. APEX1 was detected in paraffin-embedded section of human colon cancer tissue.
IHC analysis of APEX1 using anti-APEX1 antibody.APEX1 was detected in paraffin-embedded section of human lung cancer tissue.
IHC analysis of APEX1 using anti-APEX1 antibody.APEX1 was detected in paraffin-embedded section of mouse brain tissue.
IHC analysis of APEX1 using anti-APEX1 antibody.APEX1 was detected in paraffin-embedded section of rat brain tissue.
IHC, WB | |
Hamster | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IHC, WB | |
Bovine, Canine, Porcine | |
Human | |
Goat | |
Polyclonal | |
Unconjugated |
FC, ICC, IHC, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
IHC, WB | |
Canine, Human, Monkey, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
FC, IF, IHC, WB | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
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