APC10 antibody

• Catalog Number: orb345389
• Category: Antibodies
• Description: APC10 antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, IP, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids near the amino terminus of human APC10.
• Concentration: 1.50 mg/mL
• Dilution range: ELISA: 1:15,000 - 1:80,000, IHC: 1:250 - 1:1,000, IP: 1:100, WB: 1:500 - 1:1,500
• Form/Appearance: Liquid (sterile filtered)
• Purity: This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase.
• Conjugation: Unconjugated
• UniProt ID: Q9UM13
• NCBI: 150170706
• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-APC10 antibody, APC-10, APC 10, ANAPC1
• Note: For research use only
• Application notes: This affinity purified antibody has been tested for use in ELISA against the immunizing peptide and by western blotting against various cell lysates. Reactivity in other immunoassays is unknown.
• Expiration Date: 12 months from date of receipt.

Western Blot of Affinity Purified Rabbit anti-APC10. Lane 1: HeLa nuclear extract (p/n orb348671). Lane 2: HeLa whole cell lysate (p/n orb348668). Lane 3: A431 whole cell lysate (p/n orb348665). Lane 4: Jurkat whole cell lysate (p/n orb348674). Lane 5: 293 whole cell lysate (p/n orb348669). All lanes contain 20 µg of lysate or extract. Primary Antibody was used at a 1:500 dilution at room temperature for 1 h to detect human APC10 at ~26 kDa (predicted molecular weight is 21 kDa). After subsequent washing, a 1:5000 dilution of HRP conjugated Gt-a-Rabbit IgG (p/n orb347673) was used for visualization. Exposure time was 4 min.

Western Blot of Affinity Purified Rabbit Anti-APC10. Lane 1: HeLa whole cell lysate (p/n orb348668). Lane 2: HeLa Nuclear lysate (p/n orb348671). Approximately 20 µg of each lysate was loaded onto a 10% SDS-PAGE gel. Primary Antibody was used at a 1:500 dilution at room temperature for 1 h. After subsequent washing, a 1:2000 dilution of HRP conjugated Gt-a-Rabbit IgG (p/n orb347673) was used for visualization. Exposure time was 5 min. The expected molecular weight of human APC10 is 21 kDa.