AP2 alpha Antibody / TFAP2A / Activator protein 2

• Catalog Number: orb1823119
• Category: Antibodies
• Description: TATA-box binding protein (TBP) interactions with TBP-associated factors (TAFs) are required for the transcription of RNA polymerases. One particular TBP-TAF complex, snRNA-activating protein complex (SNAPC), is unusual in that it regulates basal transcription of both RNA polymerase II and III by binding specifically to a non-TATA-box proximal sequence element (PSE). SNAPC consists of five subunits of varying size. SNAPC binds to Oct-1 and TBP, which are activators of snRNA and RNA polymerases, respectively. The POU domain of Oct-1 binds to SNAPC 190 and effectively recruits SNAPC to the PSE. The cooperative binding of SNAPC and Oct-1 to their respective sequence elements is mediated by a nucleosome positioned between the two sequence elements. SNAPC 19 mediates the assembly of the subunits to form a functional SNAPC transcription regulator. SNAPC 50 (also designated PTFbeta) contains two zinc finger motifs and binds to SNAPC 43 (also designated PTFGamma) but not SNAPC 45 (PTFdelta).
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: PCRP-TFAP2A-2C2
• Tested applications: FACS, IHC-P
• Reactivity: Human
• Isotype: Mouse IgG2a
• Immunogen: Recombinant full-length human TFAP2A protein was used as the immunogen for the TFAP2A antibody.
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This TFAP2A antibody is available for research use only.
• UniProt ID: P05549
• Storage: Aliquot the TFAP2A antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human renal cell carcinoma tissue with TFAP2A antibody (clone PCRP-TFAP2A-2C2). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Western blot testing of human HeLa cell lysate with TFAP2A antibody (clone PCRP-TFAP2A-2C2). Predicted molecular weight ~47 kDa.

Flow cytometry testing of PFA-fixed human HeLa cells with TFAP2A antibody (clone PCRP-TFAP2A-2C2) followed by goat anti-mouse IgG-CF488 (blue); Red = unstained cells.

Analysis of a HuProt (TM) microarray containing more than 19, 000 full-length human proteins using TFAP2A Mouse Monoclonal (PCRP-TFAP2A-2C2). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a mAb to its intended target. A mAb is considered to specific to its intended target, if the mAb has an S-score of at least 2.5. For example, if a mAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that mAb to protein X is equal to 29.

SDS-PAGE analysis of purified, BSA-free TFAP2A antibody (clone PCRP-TFAP2A-2C2) as confirmation of integrity and purity.