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Item 1 of 4
Item 1 of 4
| Catalog Number | orb764136 |
|---|---|
| Category | Antibodies |
| Description | Rabbit polyclonal antibody to AP-1/Jun D (phospho-Ser73/100) |
| Clonality | Polyclonal |
| Tested applications | ELISA, IHC-P, WB |
| Reactivity | Human, Mouse, Rat |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human c-Jun around the phosphorylation site of Ser73. AA range:40-89 |
| Concentration | 1 mg/ml |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications. |
| Conjugation | Unconjugated |
| MW | 36 |
| Source | Rabbit |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Alternative names | Anti-JUN antibody, anti-Transcription factor AP-1 Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Western Blot analysis of various cells using Phospho-AP-1/Jun D (S73/100) Polyclonal Antibody diluted at 1:1000.
Western Blot analysis of HuvEc cells using Phospho-AP-1/Jun D (S73/100) Polyclonal Antibody diluted at 1:1000.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using c-Jun (Phospho-Ser73) Antibody. The picture on the right is blocked with the phospho peptide.
Western blot analysis of lysates from HUVEC cells treated with TNF 20ng/ml 5' and K562 cells treated with TNF 20ng/ml 5', using c-Jun (Phospho-Ser73) Antibody. The lane on the right is blocked with the phospho peptide.
