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    ANXA4 Antibody

    Catalog Number: orb1672071

    DispatchUsually dispatched within 5-10 working days
    $ 888.00
    Catalog Numberorb1672071
    CategoryAntibodies
    DescriptionANXA4 Antibody
    ClonalityRecombinant
    Clone NumberSAIC-14C-10F12
    Tested applicationsELISA, WB
    ReactivityHuman
    IsotypeIgG kappa
    ImmunogenPeptide "DEGNYLDDALVR" derived from Annexin A4 conjugated to KLH.
    Concentrationbatch dependent
    ConjugationUnconjugated
    TargetANXA4
    UniProt IDP09525
    StorageStore at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
    Buffer/PreservativesPBS with 0.02% Proclin 300.
    Alternative namesANXA4 protein
    Read more...
    NoteFor research use only
    Expiration Date12 months from date of receipt.
    ANXA4 Antibody

    Immunofluorescence staining of HepG2 cells with anti-ANXA4 (SAIC-14C-10F12). Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells permewith 0.15% Triton stained with the chimeric r version of SAIC-14C-10F12 (orb1672071) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution)- showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom orb1672071- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (3.0) followed by staining with Alexa Fluor® 488 secondary antibody.

    ANXA4 Antibody

    Western Blot using anti-ANXA4 antibody SAIC-14C-10F12. HepG2(A) (0.001 ug/ml)- A431(B) (0.001 ug/ml)- and HeLa(C) (0.01 ug/ml) cell lysates (35 ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rsion of SAIC-14C-10F12 (orb1672071)- before detection using an anti-rondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.

    ANXA4 Antibody

    Flow cytometry using the Anti-ANXA4 antibody SAIC-14C-10F12. Paraformaldehyde fixed HepG2 cells permewith 0.5% Triton were stained with anti-unknown specificity antibody (3.0; isotype control - black line) or the r version of SAIC-14C-10F12 (orb1672071 - blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

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      Bovine, Canine, Equine, Guinea pig, Mouse, Porcine, Rabbit, Zebrafish

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      Polyclonal

      Unconjugated

      100 μl
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      ICC,  IHC-Fr,  IHC-P,  WB

      Hamster, Human, Mouse, Rat

      Rabbit

      Monoclonal

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      100 μl, 50 μl, 20 μl
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      IF,  IH,  WB

      Human, Mouse, Rat

      Rabbit

      Polyclonal

      Unconjugated

      200 μl, 100 μl, 50 μl
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