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Catalog Number | orb1274548 |
---|---|
Category | Antibodies |
Description | ANXA1 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IHC, WB |
Reactivity | Human, Mouse |
Isotype | IgG |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-346 of human ANXA1 (NP_000691.1). |
Concentration | batch dependent |
Dilution range | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 38kDa |
Target | ANXA1 |
UniProt ID | P04083 |
Storage | Store at -20˚°C. Avoid freeze / thaw cycles. |
Buffer/Preservatives | Liquid |
Alternative names | ANXA1, ANX1, LPC1 Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using ANXA1 antibody (orb1274548). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Western blot analysis of extracts from normal (control) and ANXA1 knockout (KO) HeLa cells, using ANXA1 antibody (orb1274548) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunohistochemistry of paraffin-embedded human esophagus using ANXA1 antibody (orb1274548) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spleen using ANXA1 antibody (orb1274548) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using ANXA1 antibody (orb1274548). Blue: DAPI for nuclear staining.
IHC, WB | |
Bovine, Canine, Guinea pig, Rat | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, WB | |
Bovine, Canine, Human, Mouse, Porcine, Rat | |
Goat | |
Polyclonal | |
Unconjugated |
ELISA, WB | |
Bovine, Canine | |
Human, Mouse, Porcine, Rat | |
Goat | |
Polyclonal | |
Unconjugated |
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