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Catalog Number | orb1246601 |
---|---|
Category | Antibodies |
Description | ANXA1 Antibody |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, WB |
Predicted Reactivity | Bovine, Canine |
Reactivity | Human, Mouse, Porcine, Rat |
Immunogen | The immunogen for this antibody is: CQAILDETKGDYEK |
Concentration | 500 ug/mL |
Dilution range | Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 37kDa band observed in lysates of cell lines K562 and NIH3T3 (calculated MW of 38.7kDa according to Human NP_000691.1 and Mouse NP_034860.2). Approx 37kDa band observed in Rat and Pig Spleen lysates (calculated MW of 38.7kDa according to Pig NP_001157470.1). Recommended concentration: 1-3ug/ml. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Target | ANXA1 |
UniProt ID | P04083 |
NCBI | NP_000691.1 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | annexin I, ANXA1, annexin A1, ANX1, LPC1, annexin Read more... |
Note | For research use only |
Application notes | Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 37kDa band observed in lysates of cell lines K562 and NIH3T3 (calculated MW of 38.7kDa according to Human NP_000691.1 and Mouse NP_034860.2). Approx 37kDa band observed in Rat and Pig Spleen lysates (calculated MW of 38.7kDa according to Pig NP_001157470.1). Recommended concentration: 1-3ug/ml. |
Expiration Date | 12 months from date of receipt. |
orb1246601 (1 ug/ml) staining of K562 (A) and NIH3T3 (B) lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence
orb1246601 (1 ug/ml) staining of Rat Spleen lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
orb1246601 (1 ug/ml) staining of Pig Spleen lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
orb1246601 (4 ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.
orb1246601 (4 ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.
IHC, WB | |
Bovine, Canine, Guinea pig, Rat | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, WB | |
Bovine, Canine, Human, Mouse, Porcine, Rat | |
Goat | |
Polyclonal | |
Unconjugated |
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