Antibody Sampler Kit

• Catalog Number: orb342266
• Category: Assays and Kits
• Description: Antibody Sampler Kit
• Tested applications: ELISA, WB
• Immunogen: VDAC, GAPDH, alpha-Tubulin, HDAC-1, beta-Actin
• Dilution range: ELISA: 1:5,000 - 1:25,000, WB: 1:500 - 1:5,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: All loading control antibodies included in this kit - VDAC/Porin Antibody, GAPDH Antibody, alpha-Tubulin Antibody, HDAC-1 Antibody and beta-Actin Antibody are affinity purified against there respective targets.
• Conjugation: Unconjugated
• Storage: Store vials at -20° C or below prior to opening. The primary antibodies contain a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Buffer/Preservatives: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Alternative names: Loading Control Antibody Sampler Kit, Rabbit Anti-
• Note: For research use only
• Application notes: Each kit contains one unit of the following products: Anti-VDAC/Porin (RABBIT) Antibody (25 µL) Anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (RABBIT) Antibody (25 µL) Anti-alpha-Tubulin (RABBIT) Antibody (25 µL) Anti-HDAC-1 (RABBIT) Antibody (25 µL) Anti-beta Actin (RABBIT) Antibody (25 µL) Anti-RABBIT IgG (H&L) (GOAT) Antibody Peroxidase Conjugated (2 mg)
• Expiration Date: Please enquire.

Western Blot of Rabbit Anti-Alpha Tubulin Antibody. Lane 1: whole cell lysates from mouse brain. Lane 2: rat brain. Lane 3: A431 cells. Lane 4: Jurkat cells. Lane 5: HeLa cells. Load: 35 µg per lane. Primary antibody: Alpha Tubulin antibody at 1:1200 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~50 kDa corresponding to alpha tubulin (arrowhead). Other band(s): none.

Western Blot of Rabbit Anti-Beta Actin Antibody. Lane 1: molecular weight. Lane 2: human embryonic kidney 293. Lane 3: human lung carcinoma A549. Lane 4: mouse brain. Load: 35 µg per lane. Primary antibody: Beta Actin antibody at 1:1500 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~42 kDa corresponding to beta Actin (arrowhead). Other band(s): none.

Western Blot of Rabbit Anti-HDAC-1 Antibody. Lane 1: 293 whole cell lysate. Lane 2: none. Load: 35 µg per lane. Primary antibody: HDAC-1 antibody at 1:3500 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~65 kDa corresponding to human HDAC1. Other band(s): none.

Western Blot of Rabbit Anti-HDAC-1 Antibody. Lane 1: LNCaP prostate cancer cells. Lane 2: none. Load: 50 µg per lane. Primary antibody: HDAC-1 antibody at 1:1000 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 55 kDa for HDAC-1. Other band(s): none.

Western Blot of Rabbit Anti-VDAC/Porin Antibody. Lane 1: rat heart whole cell lysate. Lane 2: none. Load: 35 µg per lane. Primary antibody: VDAC/Porin antibody at 1:1200 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~32 kDa corresponding to VDAC/Porin (arrowhead). Other band(s): none.