Anti-Von Willebrand Factor/VWF Antibody

• Catalog Number: orb234319
• Category: Antibodies
• Description: Anti-Von Willebrand Factor/VWF Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived mouse VWF recombinant protein (Position: M1304-E1452).
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 309 kDa
• UniProt ID: Q8CIZ8
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: von Willebrand factor; vWF; von Willebrand antigen
• Note: For research use only
• Application notes: Western blot, 0.1-0.5μg/ml, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat Immunofluorescence, 2μg/ml, Rat. Add 0.2ml of distilled water will yield a concentration of 500ug/ml
• Expiration Date: 12 months from date of receipt.

Anti-VWF Picoband antibody, IHC(P): Mouse Liver Tissue.

Anti-VWF Picoband antibody, Western blotting. All lanes: Anti VWF at 0.5 ug/ml. WB: Mouse Lung Tissue Lysate at 50 ug. Predicted bind size: 309 KD. Observed bind size: 309 KD.

Anti-VWF Picoband antibody, Western blotting. All lanes: Anti VWF at 0.5 ug/ml. WB: Recombinant Mouse VWF Protein 0.5 ng. Predicted bind size: 37 KD. Observed bind size: 37 KD.

IF analysis of VWF and alpha-Smooth Muscle Actin using anti-VWF antibody and anti-alpha-Smooth Muscle Actin antibody. VWF and alpha-Smooth Muscle Actin a paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/mL rabbit anti-VWF antibody and mouse anti-alpha-Smooth Muscle Actin Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG and Cy3 Conjugated Goat Anti-Mouse IgG were used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

IF analysis of VWF using anti-VWF antibody. VWF was detected in paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/mL rabbit anti-VWF Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

IHC analysis of VWF using anti-VWF antibody. VWF was detected in paraffin-embedded section of rat lung tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml rabbit anti-VWF Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.