Anti-Von Willebrand Factor/VWF Antibody

• Catalog Number: orb182394
• Category: Antibodies
• Description: Anti-Von Willebrand Factor/VWF Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IHC, IHC-Fr, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human VWF recombinant protein (Position: R2535-K2813). Human VWF shares 79% amino acid (aa) sequence identity with mouse VWF.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 309 kDa
• UniProt ID: P04275
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: von Willebrand factor; vWF; von Willebrand antigen
• Note: For research use only
• Application notes: Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml Immunohistochemistry (Frozen Section), 0.5-1μg/ml Immunocytochemistry, 0.5-1μg/ml Flow Cytometry (Fixed), 1-3μg/1x106 cells. Add 0.2ml of distilled water will yield a concentration of 500ug/ml
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of A431 cells using anti-VWF antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of VWF using anti-VWF antibody.Lane 1:HT1080 Cell.

IHC analysis of VWF using anti-VWF antibody.VWF was detected in paraffin-embedded section of Human Lung Cancer Tissue.

IHC analysis of VWF using anti-VWF antibody.VWF was detected in frozen section of human placenta tissue.