Anti-Vinculin VCL Antibody (Monoclonal, hVIN-1)

• Catalog Number: orb18279
• Category: Antibodies
• Description: Anti-Vinculin VCL Antibody (Monoclonal, hVIN-1). Tested in IF, IHC-F, ICC, WB applications. This antibody reacts with Chicken, Human, Monkey, Mouse, Rat.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: hVIN-1
• Tested applications: ICC, IF, IHC-Fr, WB
• Reactivity: Gallus, Human, Monkey, Mouse, Rat
• Isotype: Mouse IgG1
• Immunogen: Human vinculin, purified from uterus.
• Antibody Type: Primary Antibody
• Concentration: Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 124 kDa
• UniProt ID: P85972
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Vinculin; Metavinculin; Vcl
• Note: For research use only
• Application notes: Western blot, 1-2μg/ml, Human, chicken, monkey, mouse, rat Immunohistochemistry (Frozen Section), 2-4μg/ml, Human, chicken, mouse, rat, - Immunocytochemistry/Immunofluorescence, 2μg/ml, Human. Add 1ml of PBS buffer will yield a concentration of 100ug/ml
• Expiration Date: 12 months from date of receipt.

WB analysis of Vinculin using anti-Vinculin antibody.Lane 1:human HeLa cell; 2:human HepG2 cell; 3:monkey COS-7 cell; 4:human U-87MG cell; 5:rat heart tissue; 6:rat PC-12 cell; 7:mouse heart tissue; 8:mosue NIH/3T3 cell.

IF analysis of Vinculin using anti-Vinculin antibody.Vinculin was detected in immunocytochemical section of human mammary cancer cell.

IHC analysis of Vinculin using anti-Vinculin antibody.Vinculin was detected in frozen section of human placenta tissues.

IHC analysis of Vinculin using anti-Vinculin antibody.Vinculin was detected in frozen section of human placenta tissues.