Anti-Villin Antibody

• Catalog Number: orb705480
• Category: Antibodies
• Description: Mouse monoclonal antibody to Villin
• Species/Host: Mouse
• Clonality: Monoclonal
• Tested applications: IH, WB
• Reactivity: Human
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within human Villin. The exact sequence is proprietary.
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:500-1:1000, IHC-P: 1:100-1:300
• Form/Appearance: Mouse IgG2b. Liquid in PBS containing 50% glycerol, 0.2% rAlbumin and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: VIL1
• Entrez: 7429
• UniProt ID: P09327
• Source: Mouse
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Mouse IgG2b. Liquid in PBS containing 50% glycerol, 0.2% rAlbumin and 0.01% sodium azide.
• Alternative names: Anti-VIL antibody, anti-Villin-1 antibody
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Villin expression in HepG2 (A), SW480 (B) whole cell lysates. (Predicted band size: 92 kD; Observed band size: 92 kD)

Immunohistochemical analysis of Villin staining in human appendix formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunohistochemical analysis of Villin staining in human colon formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.