Anti-UBE2I [SAIC-36A-9]

• Catalog Number: orb1089980
• Category: Antibodies
• Description: Rabbit monoclonal antibody to UBE2I
• Species/Host: Rabbit
• Clonality: Monoclonal
• Clone Number: SAIC-36A-9
• Tested applications: ELISA, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: The original antibody was raised against the KLH-conjugated peptide "DHPFGFVAVPTK", derived from the SUMO-conjugating enzyme UBC9.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: UBE2I
• UniProt ID: P63279
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: SUMO-conjugating enzyme UBC9; UBC9; UBCE9
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometry using the Anti-UBE2I antibody SAIC-36A-9. Paraformaldehyde fixed Jurkat cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (orb256458; isotype control, black line) or the rabbit IgG version of SAIC-36A-9 (orb1089980, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of Jurak cells with anti-UBE2I SAIC-36A-9. Immunofluorescence analysis of paraformaldehyde fixed Jurak cells on Shi-fix™ cover-slips stained with the chimeric rabbit IgG version of SAIC-36A-9 (orb1089980) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb1089980, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.