Anti-Transgelin-2 Antibody

• Catalog Number: orb341173
• Category: Antibodies
• Description: Rabbit polyclonal antibody to TAGLN2
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant full length protein of human Transgelin-2
• Dilution range: WB: 1:500-1:2000, IF/ICC: 1:50-1:200
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: TAGLN2
• Entrez: 21346, 8407, 304983
• UniProt ID: Q5XFX0, P37802, Q9WVA4
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: KIAA0120; Transgelin-2; Epididymis tissue protein
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Transgelin-2 expression in HEK293T (A), Hela (B), THP1 (C), MCF7 (D), SKOV3 (E), mouse lung (F) whole cell lysates. (Predicted band size: 22; 24 kD; Observed band size: 22 kD)

Immunofluorescent analysis of Transgelin-2 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.