Anti-TRAAK [13E9]

• Catalog Number: orb1906351
• Category: Antibodies
• Description: Anti-TRAAK [13E9]
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 13E9
• Tested applications: ELISA, WB
• Reactivity: Human
• Isotype: IgG1
• Immunogen: The original antibody was generated by immunizing mice with recombinant human TRAAK.
• Concentration: 1 mg/ml
• Conjugation: Unconjugated
• Target: TRAAK
• UniProt ID: Q9NYG8
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: KCNK4; TRAAK; Potassium channel subfamily K member
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence staining of HepG2 cells with anti-TRAAK antibody 13E9. Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of 13E9 (orb1906350) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, orb1906350, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.

Western blot using anti-TRAAK antibody 13E9. Kelly cells lysate (35 µg protein in RIPA buffer) was resolved via SDS-PAGE, and the subsequent blot was probed with the chimeric rabbit version of 13E9 (orb1906350) at 1 µg/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1 hour was used, and proteins were detected by chemiluminescence.