Anti-TNF alpha [CDP 571 (Humicade)]

• Catalog Number: orb348870
• Category: Antibodies
• Description: Human monoclonal antibody to TNF alpha
• Species/Host: Human
• Clonality: Monoclonal
• Clone Number: CDP 571 (Humicade)
• Tested applications: ELISA
• Reactivity: Human
• Isotype: IgG4
• Immunogen: Human TNF-alpha
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: TNF alpha
• UniProt ID: P01375
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: TNFA; Tumour necrosis factor superfamily member 2;
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunohistochemical analysis of rat liver using anti-TNFalpha antibody. Formalin fixed rat liver slices were stained with a orb348870 at 5 µg/ml.

Western Blot using anti-TNFalpha antibody. Rat liver (lane 1), rat spinal cord (lane 2), mouse testis (lane 3), rat colon (lane 4) and human thyroid tumour (lane 5) samples were resolved on a 10% SDS PAGE gel and blots probed with orb348870 at 1 µg/ml before being detected by a secondary antibody. The expected band size for TNFalpha is 26 kDa, however dimers and other higher order complexes are well described in the literature too. orb348870 successfully detected human and rat TNF alpha.