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Anti-TGF beta 2 Antibody

Catalog Number: orb1473837

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb1473837
CategoryAntibodies
DescriptionMouse monoclonal antibody to TGF beta 2
Species/HostMouse
ClonalityMonoclonal
Tested applicationsIF, IH, WB
ReactivityHuman
ImmunogenRecombinant fusion protein of human TGF beta 2. The exact sequence is proprietary.
Dilution rangeWB (1/500 - 1/1000), IH (1/50 - 1/200), IF/IC (1/10 - 1/50)
Form/AppearanceMouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
ConjugationUnconjugated
TargetTGFB2
Entrez7042
UniProt IDP61812
SourceMouse
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesMouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternative namesTransforming growth factor beta-2; TGF-beta-2; BSC
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NoteFor research use only
Expiration Date12 months from date of receipt.
Anti-TGF beta 2 Antibody

Western blot analysis of TGF beta 2 expression in 293 (A), K562 (B) whole cell lysates. (Predicted band size: 47 kD; Observed band size: 50 kD)

Anti-TGF beta 2 Antibody

Immunohistochemical analysis of TGF beta 2 staining in human kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-TGF beta 2 Antibody

Immunofluorescent analysis of TGF beta 2 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

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