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| Catalog Number | orb182397 |
|---|---|
| Category | Antibodies |
| Description | Goat polyclonal antibody to tdTomato (red fluorescent protein). tdTomato protein is derived from DsRed, an engineered red fluorescent protein from so-called disc corals of the genus Discosoma. It is a genetic fusion of two copies of the dTomato gene, which has been specifically designed for low aggregation |
| Species/Host | Goat |
| Clonality | Polyclonal |
| Tested applications | ELISA, IF, IHC-Fr, IHC-P, WB |
| Reactivity | Other |
| Isotype | IgG |
| Immunogen | Antigen: Purified recombinant peptide produced in E. coli.. Antigen Sequence: MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAEGRHSTGGMDELYK |
| Concentration | 1 mg/ml |
| Dilution range | WB 1:500-1:2,000 IHC (F) 1:50-1:500 IHC (P) 1:50-1:500 IF 1:50-1:500 |
| Purity | This antibody is epitope-affinity purified from goat antiserum |
| Conjugation | Unconjugated |
| MW | 55 kDa |
| Target | Red Fluorescent Protein |
| Protein Sequence | MEQKLISEEDLGTISTMVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAEGRHSNGGMDEVYK |
| RRID | AB_2687917 |
| Storage | For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours |
| Buffer/Preservatives | PBS, 20% glycerol and 0.05% sodium azide |
| Alternative names | Cherry fluorescent protein, DsRed, mCherry, red fl Read more... |
| Note | For research use only |
| Application notes | In 293HEK cells transfected with cds plasmid detects a band of 55 kDa by Western blot. It also detects tdTomato in brain sections by IHC. This antibody (AB8181) is specific for tdTomato and mCherry proteins. It does not cross-react to GFP (green fluorescent protein) |
| Expiration Date | 12 months from date of receipt. |
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Yang, Yang et al. Derivation of Pluripotent Stem Cells with In Vivo Embryonic and Extraembryonic Potency Cell, 169, 243-257.e25 (2017)
Applications
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Xiangyi Ke 1, Benjamin van Soldt 2, Lukas Vlahos 3, Yizhuo Zhou 4, Jun Qian 5, Joel George 6, Claudia Capdevila 7, Ian Glass 8, Kelley Yan 7, Andrea Califano 9, Wellington V Cardoso Morphogenesis and regeneration share a conserved core transition cell state program that controls lung epithelial cell fate Dev Cell ., (2024)
Siwen Chen # 1 2, Yingxin Lin # 3 4 5, Hao Yang # 6, Zihao Li 1 2, Sifang Li 1 2, Dongying Chen 7, Wenjun Hao 1 2, Shuai Zhang 1 2, Hua Chao 1 2, Jingyu Zhang 1 2, Jianru Wang 1 2, Zemin Li 1 2, Xiang Li 1 2, Zhongping Zhan 7, Hui Liu A CD26+ tendon stem progenitor cell population contributes to tendon repair and heterotopic ossification Nat Commun, (2025)
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Yinshan Fang 1, Sanny S W Chung 1, Le Xu 2, Chenyi Xue 3, Xue Liu 4, Dianhua Jiang 4, Rongbo Li 2, Yohei Korogi 2, Ke Yuan 5, Anjali Saqi 6, Hanina Hibshoosh 6, Yuefeng Huang 7, Chyuan-Sheng Lin 8, Tatsuya Tsukui 9, Dean Sheppard 9, Xin Sun 10, Jianwen Que RUNX2 promotes fibrosis via an alveolar-to-pathological fibroblast transition Nature, (2025)
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Reactivity
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IF, IHC
Reactivity
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Li, Hui et al. Identification of novel B-1 transitional progenitors by B-1 lymphocyte fate-mapping transgenic mouse model Bhlhe41 dTomato-Cre Front Immunol, 13, 946202 (2022)
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Gu, Bin et al. Live imaging YAP signalling in mouse embryo development Open Biol, 12, 210335 (2022)
Western blot analysis of HEK293 cell line lysate using tdTomato antibody.
Confocal immunofluoroscence analysis of HEK293 cells using tdTomato antibody.
