Anti-TAG-72 [Minretumomab (CC49 )]

• Catalog Number: orb669751
• Category: Antibodies
• Description: Mouse monoclonal antibody to TAG-72
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: Minretumomab (CC49 )
• Tested applications: FC, IHC, RIA, WB
• Reactivity: Human
• Isotype: IgG1
• Immunogen: This antibody was raised by immunising BALB/c mice with TAG-72 purified from LS-174T human carcinoma xenograft.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: TAG-72
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: CA 72-4; Tumor-associated antigen 72; TAG72; Tumor
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometry using the Anti-TAG-72 antibody Minretumomab (CC49). Paraformaldehyde fixed Jurkat cells were stained with anti-unknown specificity antibody (orb256449; isotype control, black line) or the mouse IgG1 version of Minretumomab (CC49) (orb669751, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Flow-cytometry using the anti-TAG-72 antibody Minretumomab (CC49). Jurkat cells were stained with anti-unknown IgG antibody (orb256449; isotype control, black line) or the mouse IgG-chimeric version of Minretumomab (CC49) (orb669751, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of Jurkat cells using anti-TAG-72 Minretumomab (CC49). Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells stained with the chimeric mouse IgG version of Minretumomab (CC49) (orb669751) at 10 µg/ml followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb669758, DAPI, merged channels and an isotype control. The isotype control was stained with anti-unknown antibody (orb256449) followed by Alexa Fluor® 488 secondary antibody.