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Anti-SPARCL1 Antibody
Catalog Number: orb443129
| Catalog Number | orb443129 |
|---|---|
| Category | Antibodies |
| Description | Anti-SPARCL1 Antibody. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | IHC, WB |
| Reactivity | Human, Mouse, Rat |
| Isotype | Rabbit IgG |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human SPARCL1, identical to the related mouse and rat sequences. |
| Antibody Type | Primary Antibody |
| Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
| Form/Appearance | Lyophilized |
| Conjugation | Unconjugated |
| MW | 62 kDa |
| UniProt ID | Q14515 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | SPARC-like protein 1; High endothelial venule prot Read more... |
| Note | For research use only |
| Application notes | Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml. Add 0.2ml of distilled water will yield a concentration of 500ug/ml |
| Expiration Date | 12 months from date of receipt. |
IHC analysis of SPARCL1 using anti-SPARCL1 antibody. SPARCL1 was detected in paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-SPARCL1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IHC analysis of SPARCL1 using anti-SPARCL1 antibody. SPARCL1 was detected in paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-SPARCL1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
Western blot analysis of SPARCL1 using anti-SPARCL1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: human SHG-44 whole cell lysates, Lane 3: human MDA-MB-231 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse smooth muscle tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPARCL1 antigen affinity purified polyclonal antibody at 0.5 mg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SPARCL1 at approximately 62KD. The expected band size for SPARCL1 is at 75KD.
