Anti-SPARC Antibody

• Catalog Number: orb215523
• Category: Antibodies
• Description: Rabbit polyclonal antibody to SPARC
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Human, Mouse, Rat, Sheep, Zebrafish
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human SPARC. The exact sequence is proprietary.
• Dilution range: WB: 1-500-1-1000
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: SPARC
• Entrez: 6678, 24791, 20692
• UniProt ID: P16975, P07214, P09486
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti ON antibody, anti SPARC antibody, anti Baseme
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of SPARC expression in A549 (A), mouse kidney (B), mouse brain (C), rat brain (D) whole cell lysates. (Predicted band size: 34 kD; Observed band size: 34 kD)

Immunohistochemical analysis of SPARC staining in mouse muscle formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.