Anti-SMYD3 Antibody

• Catalog Number: orb412581
• Category: Antibodies
• Description: Rabbit polyclonal antibody to SMYD3
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse
• Immunogen: Recombinant full length protein of human SMYD3
• Dilution range: WB: 1:500-2000, IF/ICC: 1:50-200
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: SMYD3
• Entrez: 69726, 64754
• UniProt ID: Q9H7B4, Q9CWR2
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti-ZMYND1 antibody, anti-ZNFN3A1 antibody, anti-
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of SMYD3 expression in Jurkat (A), MCF7 (B), Hela (C), mouse heart (D) whole cell lysates. (Predicted band size: 29; 42; 49 kD; Observed band size: 49 kD)

Immunofluorescent analysis of SMYD3 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.