Anti-Secretin Receptor Antibody

• Catalog Number: orb1422461
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Secretin Receptor.
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: SCTR
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Rat
• Dilution range: WB: WB (1/500 - 1/1000), IF/IC (1/100 - 1/500), IF: WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)
• Conjugation: Unconjugated
• UniProt ID: P47872
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Secretin receptor; SCT-R
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescent analysis of Secretin Receptor staining in SHSY5Y Cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4°C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Western blot analysis of Secretin Receptor expression in HEK293T (A), Hela (B), H460 (C), mouse lung (D), mouse kidney (E), rat kidney (F) whole cell lysates.