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Catalog Number | orb154406 |
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Category | Antibodies |
Description | Mouse monoclonal antibody to SCIMP which also known as Nvl, is a palmitoylated transmembrane adaptor protein expressed in professional antigen presenting cells, most prominently in the lymph nodes and spleen. It encodes a transmembrane adaptor protein that is expressed in antigen-presenting cells and is localized in the immunologic synapse. |
Clonality | Monoclonal |
Clone Number | NVL-07 |
Tested applications | FC, ICC, IP, WB |
Reactivity | Human |
Isotype | Mouse IgG2a |
Immunogen | Recombinant intracellular part of human SCIMP |
Antibody Type | Primary Antibody |
Concentration | 1 mg/ml |
Purity | Purified by protein-A affinity chromatography. |
Conjugation | Unconjugated |
Target | SCIMP |
Entrez | 388325 |
UniProt ID | Q6UWF3 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
Alternative names | Anti-NVL antibody Read more... |
Note | For research use only |
Application notes | Flow cytometry: Intracellular staining. |
Expiration Date | 12 months from date of receipt. |
Flow cytometry analysis (intracellular staining) of SCIMP in a population of HEK-293T-SCIMP transfectants using monoclonal antibody (clone NVL-07, purified).The staining pattern reflects heterogeneity in the cell population regarding transfection efficiency.
Western blotting analysis of human SCIMP using mouse monoclonal antibody NVL-07 on lysates of human SCIMP transfectants under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. SCIMP was detected around 17-22 kDa.