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Anti-RANBP6 Antibody

Catalog Number: orb215125

DispatchUsually dispatched within 5-10 working days
$ 140.00
Catalog Numberorb215125
CategoryAntibodies
DescriptionRabbit polyclonal antibody to RANBP6
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF, IH, WB
ReactivityHuman, Mouse, Primate, Rat
ImmunogenKLH-conjugated synthetic peptide encompassing a sequence within the center region of human RANBP6. The exact sequence is proprietary.
Antibody TypePrimary Antibody
Dilution rangeWB: 1-500-1-1000, IHC-P: 1-100-1-200, IF/ICC: 1-100-1-500, IP: 1-10-1-100
Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
ConjugationUnconjugated
TargetRANBP6
Entrez26953, 240614
UniProt IDO60518, Q8BIV3
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternative namesanti Ran-binding protein 6 antibody, anti RanBP6 a
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NoteFor research use only
Expiration Date12 months from date of receipt.
Anti-RANBP6 Antibody

Western blot analysis of RANBP6 expression in Hela (A), mouse brain (B), rat brain (C), rat testis (D) whole cell lysates. (Predicted band size: 124 kD; Observed band size: 125 kD)

Anti-RANBP6 Antibody

Immunohistochemical analysis of RANBP6 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-RANBP6 Antibody

Immunofluorescent analysis of RANBP6 staining in K562 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

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