RAD23B Antibody

SKU: orb1474301

Description

The RAD23B Antibody is suitable for IF, IHC, WB. It is a Monoclonal, Unconjugated antibody which raised against Purified recombinant human hHR23b protein fragments expressed in E.coli. Purification: The antibody was purified by immunogen affinity chromatography.

Images & Validation

• Tested Applications: IF, IHC, WB
• Dilution range: WB (1/500 - 1/1000), IH (1/50 - 1/100), IF/IC (1/50 - 1/100)
• Reactivity: Hamster, Human, Monkey, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Immunogen: Purified recombinant human hHR23b protein fragments expressed in E.coli.
• Purification: The antibody was purified by immunogen affinity chromatography.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid in PBS containing 50% glycerol, 0.5% rAlbumin and 0.02% sodium azide, pH 7.3.
• Disclaimer: For research use only

Alternative Names

UV excision repair protein RAD23 homolog B; HR23B; hHR23B; XP-C repair-complementing complex 58 kDa protein; p58

Western blot analysis of RAD23B expression in A431 (A), K562 (B), Jurkat (C), C6 (D), NIH3T3 (E), Hela (F) whole cell lysates. (Predicted band size: 43 kD; Observed band size: 58 kD)

Immunohistochemical analysis of RAD23B staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.103). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of RAD23B staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.

Quick Database Links

UniProt

P54727, Q4KMA2, P54728

NCBI Gene ID

298012, 19359, 5887