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Description
Images & Validation
−| Tested Applications | FC, WB |
|---|---|
| Dilution range | WB (1/500 - 1/2000), FC (1/10 - 1/50) |
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Immunogen | Recombinant fusion protein of human RAB1B. The exact sequence is proprietary. |
| Purification | This antibody is purified through a protein G column. |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−Rab1 Antibody [orb153345]
IF, IHC-Fr, IHC-P, WB
Bovine, Canine, Donkey, Equine, Feline, Gallus, Goat, Guinea pig, Hamster, Human, Mouse, Other, Porcine, Primate, Rabbit, Rat, Sheep
Goat
Polyclonal
Unconjugated
100 μgRAB1B Antibody (C-term) [orb1936382]
IHC-P, WB
Bovine, Monkey, Porcine
Human, Mouse, Rat
Rabbit
Polyclonal
Unconjugated
100 μl, 50 μlRAB1B Rabbit Polyclonal Antibody [orb585862]
WB
Bovine, Canine, Equine, Guinea pig, Rat
Human, Mouse
Rabbit
Polyclonal
Unconjugated
100 μl

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Western blot analysis of RAB1B expression in Hela (A), A431 (B), U20S (C) whole cell lysates. (Predicted band size: 22 kD; Observed band size: 22 kD)

Flow cytometric analysis of A431 cells using Anti-RAB1B Antibody. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37 °C for 60 min. The secondary antibody Goat Anti-Mouse IgG (H&L) - AF488 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.
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Protocol Information
RAB1B Antibody (orb1473681)
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