Anti-Pyruvate Kinase Antibody

• Catalog Number: orb1474347
• Category: Antibodies
• Description: Mouse monoclonal antibody to Pyruvate Kinase
• Species/Host: Mouse
• Clonality: Monoclonal
• Tested applications: IF, IP, WB
• Reactivity: Human, Mouse, Primate
• Immunogen: Purified recombinant human PKM2 protein fragments expressed in E.coli.
• Dilution range: WB (1/500 - 1/1000), IF/IC (1/50 - 1/100), IP (1/10 - 1/50)
• Form/Appearance: Liquid in PBS containing 50% glycerol, 0.5% rAlbumin and 0.02% sodium azide, pH 7.3.
• Conjugation: Unconjugated
• Target: PKM
• Entrez: 18746, 5315
• UniProt ID: P14618, P52480
• Source: Mouse
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in PBS containing 50% glycerol, 0.5% rAlbumin and 0.02% sodium azide, pH 7.3.
• Alternative names: OIP3; PK2; PK3; PKM2; Pyruvate kinase PKM; Cytosol
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Pyruvate Kinase expression in HCT116 (A), COS7 (B), K562 (C), A549 (D), Hela (E) whole cell lysates. (Predicted band size: 58 kD; Observed band size: 58 kD)

Immunofluorescent analysis of Pyruvate Kinase staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.