Anti-PRMT5 [RAB-C136]

• Catalog Number: orb1089932
• Category: Antibodies
• Description: Human polyclonal antibody to PRMT5
• Species/Host: Human
• Clonality: Monoclonal
• Clone Number: RAB-C136
• Tested applications: ChIP, ELISA, IF
• Reactivity: Human
• Isotype: Fab fragment
• Immunogen: This antibody was obtained by recombinant antibody (rAb) phage display recognizing PRMT5 protein under non-denaturing conditions.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: PRMT5
• UniProt ID: O14744
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: PRMT5; Protein arginine N-methyltransferase 5; 72
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometry using the anti-PRMT5 antibody RAB-C136. HeLa cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (orb256458; isotype control, black line) or the rabbit IgG1 version of RAB-C136 (orb1089955, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of fixed HeLa cells with anti-PRMT5 antibody RAB-C136. Immunofluorescence analysis of paraformaldehyde fixed HeLa cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of RAB-C136 (orb1089955) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb1089955, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.