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Anti-PRKAR2A Purified
Catalog Number: orb414536
| Catalog Number | orb414536 |
|---|---|
| Category | Antibodies |
| Description | Mouse monoclonal antibody to PRKAR2A |
| Clonality | Monoclonal |
| Clone Number | Hs-36 |
| Tested applications | ICC, WB |
| Reactivity | Human |
| Isotype | Mouse IgM |
| Immunogen | Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization. |
| Antibody Type | Primary Antibody |
| Concentration | 1 mg/ml |
| Purity | Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods). |
| Conjugation | Unconjugated |
| Target | PRKAR2A |
| Entrez | 5576 |
| UniProt ID | P13861 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide |
| Alternative names | Anti-PKAR2A antibody, anti-PKR2 antibody, anti-PKA Read more... |
| Note | For research use only |
| Application notes | Immunocytochemistry: Recommended dilution: 10 μg/ml; membrane permeabilization (acetone) is essential. |
| Expiration Date | 12 months from date of receipt. |
Immunocytochemistry staining of normal human sperma with anti-PRKAR2A antibody (intracellular signal in acrosomes, green); DNA visualized by DAPI (blue).
