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Catalog Number | orb1743953 |
---|---|
Category | Antibodies |
Description | Anti-PPP3CB Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, FC, IHC, WB |
Reactivity | Human, Monkey, Mouse, Rat |
Immunogen | E.coli-derived human PPP3CB recombinant protein (Position: H36-Q524). |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 59 kDa |
UniProt ID | P16298 |
Storage | At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing. |
Note | For research use only |
Application notes | Western blot, 0.25-0.5 μg/ml, Human, Monkey, Mouse, Rat Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human Flow Cytometry (Fixed), 1-3 μg/1x10^6 cells, Human Direct ELISA, 0.1-0.5 μg/ml, Human. Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml |
Expiration Date | 12 months from date of receipt. |
Flow Cytometry analysis of U937 cells using anti-PPP3CB antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG. Unlabelled sample (Red line) was also used as a control.
WB analysis of PPP3CB using anti-PPP3CB antibody.Lane 1:human MCF-7 cell;2:monkey COS-7 cell;3:rat brain tissue;4:rat heart tissue;5:rat C6 cell;6:mouse brain tissue;7:mouse heart tissue;8:mouse NIH/3T3 cell.
IHC analysis of PPP3CB using anti-PPP3CB antibody. PPP3CB was detected in a paraffin-embedded section of human rectum adenocarcinoma tissue.
IHC analysis of PPP3CB using anti-PPP3CB antibody. PPP3CB was detected in a paraffin-embedded section of human tonsil tissue.
IHC analysis of PPP3CB using anti-PPP3CB antibody. PPP3CB was detected in a paraffin-embedded section of human urothelial carcinoma tissue.
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