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Catalog Number | orb1786066 |
---|---|
Category | Antibodies |
Description | Anti-POLR2B Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, FC, ICC, IF, WB |
Reactivity | Human, Mouse, Rat |
Immunogen | E.coli-derived human POLR2B recombinant protein (Position: E49-Q1145). |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 130 kDa |
UniProt ID | P30876 |
Storage | At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing. |
Note | For research use only |
Application notes | Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Flow Cytometry (Fixed), 1-3 μg/1x10^6 cells, Human ELISA, 0.1-0.5 μg/ml, Human. Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml |
Expiration Date | 12 months from date of receipt. |
Flow Cytometry analysis of U251 cells using anti-POLR2B antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG. Unlabelled sample (Red line) was also used as a control.
WB analysis of POLR2B using anti-POLR2B antibody.Lane 1:human A431 cell;2:human HeLa cell;3:human Ramos cell;4:human U251 cell;5:rat brain tissue;6:rat RH35 cell;7:mouse brain tissue;8:mouse NIH/3T3 cell.
IF analysis of POLR2B using anti-POLR2B antibody and anti-Beta Tubulin antibody. POLR2B was detected in immunocytochemical section of A549 cell.
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