Anti-PGP9.5 Antibody

• Catalog Number: orb1474026
• Category: Antibodies
• Description: Mouse monoclonal antibody to PGP9.5
• Species/Host: Mouse
• Clonality: Monoclonal
• Tested applications: IH, WB
• Reactivity: Human, Rat
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human PGP9.5. The exact sequence is proprietary.
• Dilution range: WB (1/500 - 1/1000), IH (1/50 - 1/200)
• Form/Appearance: Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: UCHL1
• Entrez: 29545, 7345
• UniProt ID: Q00981, P09936
• Source: Mouse
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: Ubiquitin carboxyl-terminal hydrolase isozyme L1;
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of PGP9.5 expression in 293T (A), NCIH1299 (B), PC12 (C) whole cell lysates. (Predicted band size: 24 kD; Observed band size: 25 kD)

Immunohistochemical analysis of PGP9.5 staining in human astroglioma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.