Anti-PDPK1 Antibody

• Catalog Number: orb315647
• Category: Antibodies
• Description: Rabbit polyclonal antibody to PDPK1
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Human, Mouse, Porcine, Rabbit, Rat, Sheep
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human PDPK1. The exact sequence is proprietary.
• Antibody Type: Primary Antibody
• Dilution range: WB: 1-500:1000, IHC-P: 1-100:200
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: PDPK1
• Entrez: 18607, 5170, 81745
• UniProt ID: Q9Z2A0, O15530, O55173
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti PDK1 antibody, anti 3-phosphoinositide-depend
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Ju-Mi Hwang 1, Woo-Jin Lee 1, Jae-Hwan Jo 1, Claudine Uwamahoro 1, Seung-Ik Jang 1, Eun-Ju Jung 1, Jeong-Won Bae 1, Dae-Hyun Kim 2, Jun Koo Yi 3, Jae Jung Ha 4, Dong Yep Oh 4, Woo-Sung Kwon Effects of oridonin on sperm function and the PI3K/PDK1/AKT signaling pathway: Implications for reproductive toxicity Reprod Toxicol, (2024)

PubMed: 38972361

Applications

WB

Reactivity

Porcine

Western blot analysis of PDPK1 expression in Hela (A), PC12 (B) whole cell lysates. (Predicted band size: 63 kD; Observed band size: 63 kD)

Immunohistochemical analysis of PDPK1 staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.