Anti-Paxillin (LD4 Motif) [LD4 sAB]

• Catalog Number: orb613836
• Category: Antibodies
• Description: Mouse monoclonal antibody to Paxillin (LD4 Motif)
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: LD4 sAB
• Tested applications: ELISA, WB
• Reactivity: Human
• Isotype: IgG1
• Immunogen: Peptide corresponding to human paxillin-LD4 motif (residues 260-278) fused to MBP; peptide sequence: WGGSATRELDELMASLSD.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: Paxillin (LD4 Motif)
• UniProt ID: P49023
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: PXN
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence staining of fixed HeLa cells with anti-Paxillin (LD4 Motif) antibody LD4 sAB. Immunofluorescence analysis of paraformaldehyde fixed HeLa cells on Shi-fix™ coverslips, permeabilized with 0.15% Triton and stained with the chimeric mouse IgG1 version of LD4 sAB (orb613836) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing nuclear periphery and diffuse cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb613836, DAPI, merged channels and an isotype control. The isotype control was stained with an unknown specificity antibody (orb256449) followed by Alexa Fluor® 488 secondary antibody.