Anti-Paxillin (LD2 Motif) [LD2 sAB]

• Catalog Number: orb613839
• Category: Antibodies
• Description: Rabbit monoclonal antibody to Paxillin (LD2 Motif)
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: LD2 sAB
• Tested applications: ELISA, WB
• Reactivity: Human
• Isotype: Mouse IgG
• Immunogen: Peptide corresponding to paxillin-LD2 motif (residues 141-156) fused to MBP; peptide sequence: NLSELDRLLLELNAVQHNP.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: Paxillin (LD2 Motif)
• UniProt ID: P49023
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: PXN
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot using anti-Paxillin (LD2 Motif) antibody LD2 sAB HepG2 (A), A431 (B), and HeLa (C) cell lysate samples (35 µg protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric mouse IgG1 version of LD2 sAB (orb613838) at 2 µg/ml before detection using an anti-mouse secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The predicted running size for Paxillin is 64.5 kDa. orb613838 successfully detected Paxillin in HepG2, A431, and HeLa cell lysate samples.