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Anti-PAK1/2/3 (Phospho-S144/141/139) Antibody

Catalog Number: orb393128

DispatchUsually dispatched within 5-10 working days
$ 160.00
Catalog Numberorb393128
CategoryAntibodies
DescriptionRabbit polyclonal antibody to PAK1 (Phospho-S144/141/139)
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIH, WB
ReactivityHuman, Mouse, Rat
ImmunogenKLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S144/141/139 of human PAK1/2/3 protein. The exact sequence is proprietary.
Dilution rangeWB: 1:500:1000, IHC-P: 1:100:200
Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
ConjugationUnconjugated
TargetPAK1; PAK2; PAK3
Entrez100910732, 29433, 5062, 5063, 224105, 29431, 5058, 18481
UniProt IDQ61036, Q13153, P35465, Q62829, O88643, Q8CIN4, Q64303, O75914, Q13177
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternative namesPAK1; Serine/threonine-protein kinase PAK 1; Alpha
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NoteFor research use only
Expiration Date12 months from date of receipt.
Anti-PAK1/2/3 (Phospho-S144/141/139) Antibody

Western blot analysis of PAK1/2/3 (Phospho-S144/141/139) expression in HepG2 (A), mouse brain (B) whole cell lysates. (Predicted band size: 60; 58; 62 kD; Observed band size: 68 kD)

Anti-PAK1/2/3 (Phospho-S144/141/139) Antibody

Immunohistochemical analysis of PAK1/2/3 (Phospho-S144/141/139) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.