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Anti-PAK1/2/3 Antibody

Catalog Number: orb737136

DispatchUsually dispatched within 2-3 weeks
$ 600.00
Catalog Numberorb737136
CategoryAntibodies
DescriptionRabbit polyclonal antibody to PAK1
Species/HostRabbit
ClonalityPolyclonal
Clone NumberPAK1; PAK2; PAK3
Tested applicationsIF, IHC, WB
ReactivityHuman, Mouse, Rat
Dilution rangeWB: 1/500 - 1/1000, IF: 1/50 - 1/200
ConjugationUnconjugated
UniProt IDQ13153
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Alternative namesAnti-PAK1 antibody, anti-Serine/threonine-protein
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NoteFor research use only
Expiration Date12 months from date of receipt.
Anti-PAK1/2/3 Antibody

Western blot analysis of PAK1/2/3 expression in A375 (A), A549 (B) whole cell lysates.

Anti-PAK1/2/3 Antibody

Immunohistochemical analysis of PAK1/2/3 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-PAK1/2/3 Antibody

Immunofluorescent analysis of PAK1/2/3 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4°C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

  • Anti-PAK1/2/3 Antibody [orb315643]

    IF,  IH,  WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    200 μl, 100 μl, 30 μl
  • Anti-PAK1/2/3 (Phospho-S144/141/139) Antibody [orb393128]

    IH,  WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    100 μl, 200 μl, 30 μl
  • PAK1/2/3 peptide [orb315909]

    Blocking

    > 85%

    Synthetic

    5 mg, 1 mg
  • PAK1 (Phospho-S144/141/139) Blocking Peptide [orb392830]

    Blocking

    > 85%

    Synthetic

    5 mg, 1 mg