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Anti-PAK1/2/3 Antibody

Catalog Number: orb315643

DispatchUsually dispatched within 5-10 working days
$ 140.00
Catalog Numberorb315643
CategoryAntibodies
DescriptionRabbit polyclonal antibody to PAK1/2/3
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF, IH, WB
ReactivityHuman, Mouse, Rat
ImmunogenKLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human PAK1/2/3. The exact sequence is proprietary.
Dilution rangeWB: 1-500:1000, IHC-P: 1-100:200, IF/ICC: 1-100:500
Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
ConjugationUnconjugated
TargetPAK1; PAK2; PAK3
Entrez100910732, 29433, 5062, 5063, 224105, 29431, 5058, 18481
UniProt IDQ61036, Q13153, P35465, Q62829, O88643, Q8CIN4, Q64303, O75914, Q13177
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternative namesanti PAK1 antibody, anti Serine/threonine-protein
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NoteFor research use only
Expiration Date12 months from date of receipt.
Anti-PAK1/2/3 Antibody

Western blot analysis of PAK1/2/3 expression in A375 (A), A549 (B) whole cell lysates. (Predicted band size: 60; 58; 62 kD; Observed band size: 68 kD)

Anti-PAK1/2/3 Antibody

Immunohistochemical analysis of PAK1/2/3 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-PAK1/2/3 Antibody

Immunofluorescent analysis of PAK1/2/3 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

  • Anti-PAK1/2/3 (Phospho-S144/141/139) Antibody [orb393128]

    IH,  WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    100 μl, 200 μl, 30 μl
  • Anti-PAK1/2/3 Antibody [orb737136]

    IF,  IHC,  WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    100 μl
  • PAK1/2/3 peptide [orb315909]

    Blocking

    > 85%

    Synthetic

    5 mg, 1 mg
  • PAK1 (Phospho-S144/141/139) Blocking Peptide [orb392830]

    Blocking

    > 85%

    Synthetic

    5 mg, 1 mg