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Anti-PAK1 Antibody

Catalog Number: orb338851

DispatchUsually dispatched within 5-10 working days
$ 170.00
Catalog Numberorb338851
CategoryAntibodies
DescriptionRabbit polyclonal antibody to PAK1
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF, IH, WB
ReactivityHuman, Mouse, Rat
ImmunogenKLH-conjugated synthetic peptide encompassing a sequence within the center region of human PAK1. The exact sequence is proprietary.
Dilution rangeWB: 1:500-1000, IHC-P: 1:100-200, IF/ICC: 1:100-500
Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
ConjugationUnconjugated
TargetPAK1
Entrez5058, 29431
UniProt IDP35465, Q13153, O88643
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternative namesanti Serine/threonine-protein kinase PAK 1 antibod
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NoteFor research use only
Expiration Date12 months from date of receipt.
Anti-PAK1 Antibody

Western blot analysis of PAK1 expression in Hela (A), HEK293T (B) whole cell lysates. (Predicted band size: 60 kD; Observed band size: 68 kD)

Anti-PAK1 Antibody

Immunohistochemical analysis of PAK1 staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-PAK1 Antibody

Immunofluorescent analysis of PAK1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

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