Anti-Optineurin/OPTN Antibody (monoclonal, 3D8)

• Catalog Number: orb527046
• Category: Antibodies
• Description: Anti-Optineurin/OPTN Antibody (monoclonal, 3D8). Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 3D8
• Tested applications: FC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Mouse IgG2b
• Immunogen: E.coli-derived human Optineurin recombinant protein (Position: R241-I577). Human Optineurin shares 82% amino acid (aa) sequence identity with both mouse and rat Optineurin.
• Antibody Type: Primary Antibody
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 66 kDa
• UniProt ID: Q96CV9
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Optineurin; E3-14.7K-interacting protein; FIP-2; H
• Note: For research use only
• Application notes: Western blot, 0.1-0.5μg/ml Flow Cytometry (Fixed), 1-3μg/1x106 cells. Add 0.2ml of distilled water will yield a concentration of 500μg/ml
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Optineurin/OPTN using anti-Optineurin/OPTN antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates; Lane 2: human U20S whole cell lysates; Lane 3: human placenta tissue lysates; Lane 4: human Hela whole cell lysates; Lane 5: human A549 whole cell lysates; Lane 6: human K562 whole cell lysates; Lane 7: rat C6 whole cell lysates; Lane 8: mouse Neuro-2a whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Optineurin/OPTN antigen affinity purified monoclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Optineurin/OPTN at approximately 66KD. The expected band size for Optineurin/OPTN is at 66KD.