Anti-NUMA/NUMA1 Antibody

• Catalog Number: orb1743788
• Category: Antibodies
• Description: Anti-NUMA/NUMA1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, FC, ICC, IF, IHC, WB
• Reactivity: Human, Monkey, Mouse, Rat
• Immunogen: E.coli-derived human NUMA/NUMA1 recombinant protein (Position: M1-E1954).
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 270 kDa
• UniProt ID: Q14980
• Storage: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
• Note: For research use only
• Application notes: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat, Monkey Immunohistochemistry(Paraffin-embedded Section), 1-2 μg/ml, Human Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Flow Cytometry (Fixed), 1-3 μg/1x10^6 cells, Human Direct ELISA, 0.1-0.5 μg/ml, Human. Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of RT4 cells using anti-NUMA/NUMA1 antibody(Blue line).Isotype control antibody (Green line) was rabbit IgG. Unlabelled sample (Red line) was also used as a control.

WB analysis of NUMA/NUMA1 using anti-NUMA/NUMA1 antibody.Lane 1:human HeLa cell;2:monkey COS-7 cell;3:human MCF-7 cell;4:human SH-SY5Y cell;5:human SIHA cell;6:human RT4 cell;7:rat PC-12 cell;8:mouse NIH/3T3 cell.

IF analysis of NUMA/NUMA1 using anti-NUMA/NUMA1 antibody and anti-Beta Tubulin antibody.NUMA/NUMA1 was detected in immunocytochemical section of U2OS cell.

IHC analysis of NUMA/NUMA1 using anti-NUMA/NUMA1 antibody. NUMA/NUMA1 was detected in a paraffin-embedded section of human acinar adenocarcinoma of prostate tissue.

IHC analysis of NUMA/NUMA1 using anti-NUMA/NUMA1 antibody. NUMA/NUMA1 was detected in a paraffin-embedded section of human breast cancer tissue.

IHC analysis of NUMA/NUMA1 using anti-NUMA/NUMA1 antibody. NUMA/NUMA1 was detected in a paraffin-embedded section of human esophageal squamous carcinoma tissue.

IHC analysis of NUMA/NUMA1 using anti-NUMA/NUMA1 antibody. NUMA/NUMA1 was detected in a paraffin-embedded section of human lung cancer tissue.