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Catalog Number | orb1743776 |
---|---|
Category | Antibodies |
Description | Anti-NR5A2 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, FC, IHC, WB |
Reactivity | Human |
Immunogen | E.coli-derived human NR5A2 recombinant protein (Position: K192-A508). |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 70 kDa |
UniProt ID | O00482 |
Storage | At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing. |
Note | For research use only |
Application notes | Western blot, 0.25-0.5 μg/ml, Human Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human Flow Cytometry (Fixed), 1-3 μg/1x10^6 cells, Human Direct ELISA, 0.1-0.5 μg/ml, Human. Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml |
Expiration Date | 12 months from date of receipt. |
Flow Cytometry analysis of CACO-2 cells using anti-NR5A2 antibody(Blue line).Isotype control antibody (Green line) was rabbit IgG. Unlabelled sample (Red line) was also used as a control.
WB analysis of NR5A2 using anti-NR5A2 antibody.Lane 1:human HepG2 cell;2:human A549 cell;3:human Caco-2 cell;4:human 293T cell;5:human MCF-7 cell;6:human K562 cell.
IHC analysis of NR5A2 using anti-NR5A2 antibody. NR5A2 was detected in a paraffin-embedded section of human parotid acinar cell carcinoma tissue.
IHC analysis of NR5A2 using anti-NR5A2 antibody. NR5A2 was detected in a paraffin-embedded section of human bladder urothelial carcinoma tissue.
IHC analysis of NR5A2 using anti-NR5A2 antibody. NR5A2 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue.
ELISA, WB | |
Bovine, Canine, Human, Mouse, Porcine, Rat | |
Goat | |
Polyclonal | |
Unconjugated |
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